Sedeen supports many image formats to make multimodality registration possible, such as:

  • A variety of whole slide images
  • Radiology images in DICOM format
  • Generic TIFF and JPEG-2000 images
  • Variety of small formats such as JPEG and PNG images


The image overlay system can be used for many applications including:

  • Alignment of serial sections
    • H&E with H&E
    • H&E with IHC

  • Multimodality biomarker validation
    • Protein expression and colocalization (e.g. H&E and IHC) 
    • Radiology and histology


This video shows how to register and overlay two slides.




Video Summary


First, in the File Manager panel, under the Transform tab, you may want to adjust pixel spacing and scale your images.


In Stacked Layout view:

  • Use the left mouse button to drag all images.
  • Use the right mouse button to drag a single image.


To rotate an image, hold shift while dragging with the right mouse button.